Real-Time Quantitative PCR for Human Herpesvirus 6 DNA
نویسندگان
چکیده
منابع مشابه
Frequency of Human Herpesvirus 6 (HHV-6) in Pterygium Using Real-Time PCR Based on SYBR-Green I Fluorescence
ABSTRACT Background and objectives: Pterygium is a non-cancerous growth of conjunctival tissue that can extend onto the corneal surface. The presence of some oncogenic viruses in pterygium and the neoplastic nature of these lesions led us to the postulated involvement of the viruses in the etiology of pterygium. Given the association of human herpesvirus 6 (HHV-6) with ocular disea...
متن کاملReal-time PCR for quantification of human herpesvirus 6 DNA from lymph nodes and saliva.
A real-time quantitative PCR assay has been developed to measure human herpesvirus 6 (HHV-6) DNA in biological specimens. The assay sensitivity was 10 copies of DNA per well, with a linear dynamic range of 10 to 10(7) copies of HHV-6 DNA. Intra- and interassay variations were, respectively, 0.88 and 0.8% for samples containing 10(2) DNA copies, 0.99 and 0.96% for samples containing 10(4) copies...
متن کاملDetection of human cytomegalovirus DNA by real-time quantitative PCR.
A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.
متن کاملLightCycler-based quantitative PCR for rapid detection of human herpesvirus 6 DNA in clinical material.
Locatelli et al. (6) reported on the quantitative detection of human herpesvirus 6 (HHV-6) in plasma and cell suspensions by TaqMan-based PCR. We wish to add our experience. We have developed a rapid (,30-min) assay for the simultaneous detection, quantification, and differentiation of HHV-6 variants A and B using a LightCycler (LC; Bio/Gene Ltd., Kimbolton, England). The instrument performs re...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2000
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.38.11.4042-4048.2000